For detailed instructions, refer to the Qubit® Fluorometer manual. Optional: Using the Dilution Calculator feature of the Qubit® Fluorometer, determine the stock concentration of your. The use of the Qubit® Fluorometer (corresponding to steps – and –) can be found in the user manual accompanying the instrument. Fluorometer The Qubit® Fluorometer is a stand-alone instrument that does not require a connection to a computer. 1. After unpacking the instrument, place the instrument on a flat, level, dry surface. 2. Plug one end of the supplied power cord into the Qubit® Fluorometer. To the other end attach the appropriate plugFile Size: 1MB. · Qubit ® dsDNA BR Assay Kits. For use with the Qubit. ®“Qubit Fluorometer” on page 4. ®“Qubit Fluorometer” on page 5: Qubit. Fluorometer User Guide. The Qubit® Fluorometer has an upgraded, robust design, complete with a color touch screen and intuitive user interface (Figure 2).
(15 minutes for the Qubit™ protein assay). 6. Insert the tubes in the Qubit® Fluorometer and take readings. For detailed instructions, refer to the Qubit® Fluorometer manual. 7. Optional: Using the Dilution Calculator feature of the Qubit® Fluorometer, determine the stock concentration of your original sample. MP | MAN reagents exhibit high photostability in the Qubit Fluorometer, showing Qubit ®. The Qubit® Fluorometer quantitates DNA, RNA, and protein with unprecedented accuracy, sensitivity, and simplicity. It is designed for molecular biology labs that work with precious samples that are rare or difficult to process, and applications requiring precise measurement such as Illumina library preparations and sequencing and real-time PCR.
Qubit ® dsDNA BR Assay Kits. For use with the Qubit. ®“Qubit Fluorometer” on page 4. ®“Qubit Fluorometer” on page 5: Qubit. Fluorometer User Guide. The Qubit® Fluorometer has an upgraded, robust design, complete with a color touch screen and intuitive user interface (Figure 2). The Qubit® Fluorometer is a benchtop fluorometer that can be used for the quantitation of DNA, RNA, microRNA, and protein using the highly sensitive and accurate fluorescence-based Qubit® quantitation assays. Fluorometer gives values for the Qubit dsDNA HS Assay in ng/mL. This value corresponds to the concentration after your sample was diluted into the assay tube. To calculate the concentration of your sample, use the following equation: Concentration of your sample = QF value × x where QF value = the value given by the Qubit ®.
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